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Surface Plasmon Resonance Microscopy (SPRM) is a label free analytical tool that combines the surface plasmon resonance of metallic surfaces with imaging of the metallic surface. The heterogeneity of the refractive index of the metallic surface imparts high contrast images, caused by the shift in the resonance angle. SPRM can achieve a thickness sensitivity of few tenths of nanometer and lateral resolution achieves values of micrometer scale. SPRM is used to characterize surfaces, self-assembled monolayers, multilayer films, metal nanoparticles, oligonucleotides arrays, binding and reduction reactions. Surface Plasmon polaritons are surface electromagnetic waves coupled to oscillating free electrons of a metallic surface that propagate along a metal/dielectric interface. Since polaritons are highly sensitive to small changes in the refractive index of the metallic material, it can be used as a biosensing tool that does not require labeling. SPRM measurements can be made in real-time. Wang and collaborators studied the binding kinetics of membrane proteins in single cells. The experimental setup of an SPRM can be seen in the Figure 1, where an adherent cell is grown on a gold film and placed in an inverted microscope, p-polarized light was used to create the surface plasmons on the gold film and a CCD camera was used to create the SPR image. ==History== SPR microscopy (SPRM) is also referred as SPR imaging (SPRI) by the scientists in multiple publications. The concept of classical SPR has been since 1968 but the SPR imaging technique was introduced in 1988 by Rothenhausler and Knoll. Capturing a high resolution image of low contrast samples for optical measuring techniques is a near impossible task until the introduction of SPRM technique that came into existence in the year 1988. In SPRM technique, plasmon surface polariton (PSP) waves are used for illumination. In simple words, SPRI technology is an advanced version of classical SPR analysis, where the sample is monitored without label through the use of a CCD camera. The SPRI technology with the aid of CCD camera gives advantage of recording the sensograms and SPR images, and simultaneously analyzes hundreds of interactions. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Surface plasmon resonance microscopy」の詳細全文を読む スポンサード リンク
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